rabbit anti glut 1 igg  (Bioss)

Journal: Journal of Neuroinflammation

Article Title: Scavenger receptor CD36 governs recruitment of myeloid cells to the blood–CSF barrier after stroke in neonatal mice

doi: 10.1186/s12974-022-02388-z

Figure Lengend Snippet: Accumulation of myeloid cells is attenuated in the ipsilateral CP of neonatal CD36 KO mice subjected to tMCAO. A Representative images of CPs at the level of the lateral ventricles immunostained with GLUT-1 (blue) and Iba1 (green) ipsilateral and contralateral to tMCAO in WT and CD36 KO mice at 3 h reperfusion. Scale bar = 50 µm. B Quantification of the number of Iba1 + cells in the ipsilateral and contralateral CPs in WT and CD36 KO mice at 3 h reperfusion . C–F Quantification of CD45 + CD11b + myeloid cells ( C ), CD45 + CD11b + Ly6c hi inflammatory monocytes ( D ), CD45 + 11b + Ly6c int Ly6g + neutrophils ( E ), and CD45 + 11b + Ly6c int Ly6g − patrolling monocytes ( F ) ipsilateral and contralateral to tMCAO in WT and CD36 KO mice at 3 h and 13 h reperfusion. Two-way ANOVA with post-hoc Tukey’s Multiple Comparison test was performed to compare groups with multiple independent variables ( B – F ). Dots represent individual mice. Data are shown as mean ± SD. Individual p values are listed within figures for data that are significant

Article Snippet: Double-immunofluorescence was performed on adjacent sections blocked in 10% NGS/PBST and incubated overnight in 2% NGS/PBST with rabbit anti-mouse GLUT-1 (1:500, AbCAM), rabbit anti-Iba1 (1:500, WAKO), anti-mouse Timp1 (1:200, TFS) followed by appropriate secondary antibodies purchased from Invitrogen and DAPI.

Techniques:

Journal: BMB Reports

Article Title: Cellular and regional specific changes in multidrug efflux transporter expression during recovery of vasogenic edema in the rat hippocampus and piriform cortex

doi: 10.5483/BMBRep.2015.48.6.237

Figure Lengend Snippet: Protein expression levels of IgG (heavy chain) and ABC family in the hippocampus following SE. (A) Western blot images of IgG and ABC family in the hippocampus. IgG level is peaked 3-4 days after SE. BCRP expression is decreased 4 days after SE, and recovered at 4 weeks after SE. MRP1 expression is gradually decreased 4 days-4 weeks after SE. MRP4 expression is reduced 12 h-3 days after SE, and recovered 4 days-1 week after SE, and increased 4 weeks after SE. p-GP expression is decreased until 4 days after SE, normalized to basal level 1 week after SE, and increased 4 weeks after SE. (B) Quantitative values (mean ± S.E.M) of IgG, BCRP, MRP1, MRP4, and p-GP in the hippocampus, based on Western blotting (n = 3 per each group). Significant differences from non-SE animals, *P ＜ 0.05.

Article Snippet: Sections were then incubated in a mixture of goat anti-glucose transporter-1 (GLUT-1) IgG/rabbit anti-BCRP IgG (Acris, San Diego, CA, USA, diluted 1:100; Abbiotec., San Diego, CA, USA, diluted 1:500, respectively), mouse anti-glial fibrillary acidic protein (GFAP) IgG/rabbit anti-BCRP IgG (Chemicon, Temecula, CA, USA, diluted 1:4,000; Abbiotec, San Diego, CA, USA, diluted 1:500), goat anti-GLUT-1 IgG/rabbit anti-MRP1 IgG (Abbiotec, San Diego, CA, USA, diluted 1:100), mouse anti-GFAP IgG/rabbit anti-MRP1 IgG (Chemicon, Temecula, CA, USA, diluted 1:4,000; Abbiotec, San Diego, CA, USA, diluted 1:100), rabbit anti-GLUT-1 IgG/goat anti-MRP4 IgG (Abcam, Cambridge, UK, diluted 1:200; Origene., Rockville, MD, USA, diluted 1 : 200), mouse anti-GFAP IgG/goat anti-MRP4 IgG (Chemicon, Temecula, CA, USA, diluted 1:4,000; Origene, Rockville, MD, USA, diluted 1:200), goat anti-GLUT-1 IgG/mouse anti-p-GP IgG (Abbiotec, San Diego, CA, USA, diluted 1:100) or mouse anti-GFAP IgG/rabbit anti-p-GP IgG (Chemicon, Temecula, CA, USA, diluted 1:4000; Abbiotec, San Diego, CA, USA, diluted 1:100) in PBS containing 0.3% triton X-100 overnight at room temperature.

Techniques: Expressing, Western Blot

Journal: BMB Reports

Article Title: Cellular and regional specific changes in multidrug efflux transporter expression during recovery of vasogenic edema in the rat hippocampus and piriform cortex

doi: 10.5483/BMBRep.2015.48.6.237

Figure Lengend Snippet: Protein expression levels of IgG (heavy chain) and ABC family in the PC following SE. (A) Western blot images of IgG and ABC family in the PC. IgG level is peaked 3-4 days after SE. BCRP expression is decreased 12 h-1 day after SE, recovered 3 days after SE, and increased 4 days-4 weeks after SE. MRP1 expression is gradually decreased from 12 h to 4 weeks after SE. MRP4 expression is reduced 1-4 days after SE and increased 1-4 weeks after SE. p-GP expression is decreased 3 days-1 week after SE, and increased 4 weeks after SE. (B) Quantitative values (mean ± S.E.M) of IgG, BCRP, MRP1, MRP4, and p-GP in the PC, based on Western blotting (n = 3 per each group). Significant differences from non-SE animals, *P ＜ 0.05.

Article Snippet: Sections were then incubated in a mixture of goat anti-glucose transporter-1 (GLUT-1) IgG/rabbit anti-BCRP IgG (Acris, San Diego, CA, USA, diluted 1:100; Abbiotec., San Diego, CA, USA, diluted 1:500, respectively), mouse anti-glial fibrillary acidic protein (GFAP) IgG/rabbit anti-BCRP IgG (Chemicon, Temecula, CA, USA, diluted 1:4,000; Abbiotec, San Diego, CA, USA, diluted 1:500), goat anti-GLUT-1 IgG/rabbit anti-MRP1 IgG (Abbiotec, San Diego, CA, USA, diluted 1:100), mouse anti-GFAP IgG/rabbit anti-MRP1 IgG (Chemicon, Temecula, CA, USA, diluted 1:4,000; Abbiotec, San Diego, CA, USA, diluted 1:100), rabbit anti-GLUT-1 IgG/goat anti-MRP4 IgG (Abcam, Cambridge, UK, diluted 1:200; Origene., Rockville, MD, USA, diluted 1 : 200), mouse anti-GFAP IgG/goat anti-MRP4 IgG (Chemicon, Temecula, CA, USA, diluted 1:4,000; Origene, Rockville, MD, USA, diluted 1:200), goat anti-GLUT-1 IgG/mouse anti-p-GP IgG (Abbiotec, San Diego, CA, USA, diluted 1:100) or mouse anti-GFAP IgG/rabbit anti-p-GP IgG (Chemicon, Temecula, CA, USA, diluted 1:4000; Abbiotec, San Diego, CA, USA, diluted 1:100) in PBS containing 0.3% triton X-100 overnight at room temperature.

Techniques: Expressing, Western Blot